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Shinde, R. P.
- Efficacy of Garlic Extract and Yeast for the Control of Post Harvest Diseases of Grape
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Authors
Affiliations
1 Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), IN
1 Department of Plant Pathology and Agricultural Microbiology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), IN
Source
International Journal of Plant Protection, Vol 9, No 2 (2016), Pagination: 434-438Abstract
The present investigations were carried out with collection of diseased samples, isolation of the organisms responsible for post harvest spoilage, their pathogenicity, symptomatology, morphological characters and effect of temperature on growth and sporulation in order to suggest suitable control measures in respect of yeast as a bioagent and garlic extract as a botanical in controlling post harvest fungal diseases of grape. The causal organisms were isolated from infected grape berries and recorded association of three fungal pathogens viz., Aspergillus niger, Alternaria alternata and Penicillium digitatum. The conidial size of A. niger, A. alternata and P. digitatum are measured as 4.4 μm, 42.47 x 16.37 μm and 4.33 – 4.5 μm in size (average), respectively. Use of 5 per cent yeast, (Eremothecium cymbalariae) was found effective against Aspregillus niger (both in vitro and in vivo) and 1 per cent garlic extract was found effective against Alternaria alternata (both in vitro and in vivo).Both yeast and garlic extract increases shelf-life and market quality of fruits, with increase in glossiness of fruits. Temperature 27 ±10C was found optimum for both growth and sporulation of all isolated fungal pathogens.Keywords
Grape, Yeast, Garlic Extract, Bioagents, Disease Control.
References
- Chanchaichaovivat, A., Ruenwongsa, P. and Bhinyo Panijpan (2007). Screening and identification of Yeast strains from fruits and vegetables: Potential for biological control of Postharvest chilli. Anthracnose (Colletotrichum Capsici).Institute for Innovation and Devel. of Learning process, Mahidol University, Thailand.
- Droby, S., Hofstein, R., Wilson, C.L., Wisniewski, M., Fridlender, B., Cohen, L., Weiss, B., Daus, A., Timar, D. and Chalutz, E. (1993). Interaction of antagonistic yeasts against post-harvest pathogens of apple fruit and possible mode of action. Biological Control, 3(1): 47 -52.
- Gautam, A.K., Sharma, S., Avasthi, A. and Bhadauri, R.(2011). Diversity, pahtogenicity and toxicitogy of A. niger, an important spoilage fungi. J. Micro., 6(3): 270-280.
- Joshi, B.R. and Vaidya, J.G. (2007). Isolation of fungi from deteriorating fruit and vegetables. J. Mycol. Pl. Panthol., 37(3): 604.
- Kota, V.R., Kulkarni, S. and Hedge, Y.R. (2006).Postharvest diseases of mango and their biological management. J. Pl. Dis. Sci., 1(2): 186-188.
- Maria Amalia Brunini, Archimedes Chiarelli Nishida, Lidiane Aparecida Kanesiro, Janaina Cristina Kanesiro and Vinicius Antonio Macien Junior (2011). Postharvest treatments on quality and shelf-life of ‘Pera Rio” orange.
- Mukherjee, A., Khandker, S., Islam, M.R. and Sonia, B. Shahid (2011). Efficacy of some plant extract on the mycelial growth of Colletotichum gloeosporioides. J. Bangladesh Agril.Univ., 9 (1): 43-47.
- Raja, P. (2010). Fungal properties of plant extracts against Alternaria tenuissima : new spp. Infecting egg plant in India.Indian Phytopath., 63(1): 45-46.
- Sangeetha, C.G. and Rawal, R.O. (2008). Nutritional studies of C. gloeosporioides Penz and Sacc. The instant of mango Anthracnose.World J. Agric. Sci., 4(6): 717-720.
- Senthil, R., Prabakar, K., Rajendran, L. and Karthikeyan, G.(2011). Efficacy of different biological control agents against major postharvest pathogens of grapes under room temperature storage conditions. Phytopathol. Mediterr., 50: 55-65.
- Verma, V.S. (2007). Spectrum of post harvest losses. J. Mycol.Pl. Pathol., 37(1): 175.
- Zhang, D., Lopez-Reyes, J.G., Spadara, D., Garibaldi, A., M.L.and Gullino (2010). Efficacy of yeast antagonists used individually or in combination with hot water dipping for control of postharvest brown rot of peaches. J. Pl. Dis. & Protec., 117(5): 226-232.
- Efficacy of Garlic Extract and Yeast Against Penicillium digitatum Causing Post Harvest Fruit Rot of Citrus (Citrus sisnensis L.)
Abstract Views :187 |
PDF Views:1
Authors
Affiliations
1 Department of Plant Pathology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), IN
1 Department of Plant Pathology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), IN
Source
International Journal of Plant Protection, Vol 9, No 2 (2016), Pagination: 460-463Abstract
Freshly prepared 1 per cent garlic extract and 5 per cent yeast solution were tested against Penicillium digitatum causing fruit rot (green mold ) of sweet orange both under in vitro and in vivo. 1 per cent garlic extract gives 15.7 per cent control and 5 per cent yeast gives 15 per cent control against Penicillium digitatum. Artificially inoculated fruits treated with 1 per cent G. E. were observed in good condition upto 35 days and 5 per cent yeast treated citrus fruits upto 45 days. Use of garlic extract and yeast maintains the quality of citrus fruits, prolonged the shelf-life and delayed the rottenness presence on fruits. Penicillium digitatum could grow between temperature ranges of 20°C to 45°C, however, optimum temperature was 27 ± 1°C at which growth and sporulation of the fungus was maximum.Keywords
Citrus, Penicillium digitatum, Garlic Extract, Yeast.References
- Ankris, S. and Mirelman, A. (1999). Antimicrobial properties of allicin from garlic. Microbes Infect., 2 : 125-129.
- Anonymous (2011). Indian horticulture data base. National Horticulture Board Publication.
- Capdeville, G.D., Manoel, S. J., Jansen Santos, Mirand, S.D.P., Caetano, A.R. and Torres, F., (2006). Selection and testing of epiphytic yests to control anthracnoste in post-harvest of papaya fruit. Brazil. Embrapa Genetic Res. & Biotech. Df : 770-900.
- Chanchaichaovivat, A., Ruenwongsa, P. and Bhinyo Panijpan (2007). Screening and identification of yeast strains from fruits and vegetables: Potential for biological control of postharvest chilli. Anthracnose (Colletotrichum Capsici). Institute for Innovation and Devel. of Learning process, Mahidol University, Thailand.
- Chowdhury, M.N.A. and Rahin, M.A. (2009). Integrated crop management to control Anthracnose (Colletotrichum gloeosporioides) of mango. J. Agric. Rural Dev., 7(1 & 2): 115-120.
- Dan He, Xiao – Dong Zhong, Yuan-Ming Yin, Ping Sun, Hong and Yin Zhang (2003). Yeast application for controlling apple post harvest disease associated with Penicillium expansum Bot. Bull. Acad. Sin., 44: 211-216.
- Maria Amalia Brunini, Archimedes Chiarelli Nishida, Lidiane Aparecida Kanesiro, Janaina Cristina Kanesiro and Vinicius Antonio Macien (2011). Postharvest treatments on quality and shelf-life of ‘Pera Rio” orange.
- Yadav, B.P. and Ojaha, K.L. (1998). Management of leaf blight of egg plant using fungicide and plant extracts. I. Appl. Biol., 8 : 57-60.
- Zhang, D., Lopez-Reyes, J.G., Spadara, D., Garibaldi, A. and Gullino, M.L. (2010). Efficacy of yeast antagonists used individually or in combination with hot water dipping for control of postharvest brown rot of peaches. J. Pl. Dis & Protec., 117(5): 226-232.
- Zhulong Chan and Shiping Tian (2005). Interaction of antagonist yeast against post harvest pathogens of apple fruit and possible mode of action. Postharvest Bio. & Tech., 36: 215 -223.
- In Vivo Efficacy of Garlic Extract and Yeast for the Control of Post Harvest Diseases of Mango and Papaya
Abstract Views :332 |
PDF Views:1
Authors
Affiliations
1 Department of Plant Pathology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), IN
1 Department of Plant Pathology, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar (M.S.), IN
Source
International Journal of Plant Protection, Vol 9, No 2 (2016), Pagination: 632-634Abstract
The present investigations were carried out with collection of diseased samples, isolation of the organisms responsible forpost harvest spoilage, their pathogenicity, symptomatology and morphological characters in order to suggest suitable control measures in respect of yeast as a bioagent and garlic extract as a botanical in controlling post harvest fungal diseases of mango and papaya. The causal organisms was isolated from infected fruits and recorded association of four fungal pathogens viz., Aspergillus niger, Colletotrichum gloeosporioides, Alternaria alternata, and Rhizopus stolonifer. The pathogenicity of all pathogens was proved by artificial inoculation method. The conidial size of A. niger, A. alternata, , C. gloeosporioides, and R. stolonifer measured as 4.4 μm, 42.47 x 16.37 μm, 9.50 – 18.56 x 2.68 - 6.72 μm and 125 x 294.1 μm in size (average), respectively. Use of 5 per cent yeast, (Eremothecium cymbalariae) was found effective against Aspregillus niger and Colletotrichum gloeosporioides .1 per cent garlic extract was found effective against Colletotrichum gloeosporioides and Alternaria alternata, but not much effective against Alternaria alternata on papaya fruits. Both yeast and garlic extract increases shelf-life and market quality of fruits, with increase in glossiness of fruits.Keywords
Mango, Papaya, Post Harvest, Garlic Extract, Yeast.References
- Barnett, H.C. and Bary, B. (1972). Illustrated genera of imperfect fungi. 4thEd. APS Press, Paul. MN : 188-191pp.
- Chanchaichaovivat, A., Ruenwongsa, P. and Bhinyo Panijpan (2007). Screening and identification of yeast strains from fruits and vegetables: Potential for biological control of Postharvest chilli. Anthracnose (Colletotrichum Capsici).Institute for Innovation and Devel. of Learning process, Mahidol University, Thailand.
- Droby, S., Hofstein, R., Wilson, C.L., Wisniewski, M., Fridlender, B., Cohen, L., Weiss, B., Daus, A., Timar, D. and Chalutz, E. (1993). Interaction of antagonistic yeasts against post-harvest pathogens of apple fruit and possible mode of action. Biological Control, 3(1): 47 -52.
- Jadeja, R.B. and Vaishnav, M.V. (2000). Post harvest fruit rot in mango Co. Kesar”. Indian Phytopath., 53(4) : 492.
- Kota, V.R., Kulkarni, S. and Hedge, Y.R. (2006).Postharvest diseases of mango and their biological management. J. Pl. Dis. Sci., 1(2) : 186-188.
- Patil, V.V. (2001). Studies on pathogen associated with fruit drop of mango. M.Sc. (Ag.) Thesis, Mahatma Phule Krishi Vidyapeeth, Rahuri, Ahmednagar, M.S. (INDIA).
- Senthil, R., Prabakar, K., Rajendran, L. and Karthikeyan, G. (2011). Efficacy of different biological control agents against major postharvest pathogens of grapes under room temperature storage conditions. Phytopathol. Mediterr., 50 : 55-65.
- Singh, V.P. and Sumbali, G. (2000). Natural incidences of oxygenic Aspergillus flavus strain on the surface of per-harvest jujube fruits. Indian Paytopath., 53(4) : 404-406.
- Zhang, D., Lopez-Reyes, J.G., Spadara, D., Garibaldi, A. and Gullino, M.L. (2010). Efficacy of yeast antagonists used individually or in combination with hot water dipping for control of postharvest brown rot of peaches. J. Pl. Dis & Protec., 117 (5) : 226-232.
- Zhulong, Chan and Shiping, Tian (2005). Interaction of antagonist yeast against post harvest pathogens of apple fruit and possible mode of action. Postharvest Bio. & Tech., 36: 215 -223.